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KMID : 1098420160240010055
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Korean Journal of Medicinal Crop Science
2016 Volume.24 No. 1 p.55 ~ p.61
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Morphological Characteristics and Genetic Diversity Analysis of Platycodon grandiflorum (Jacq.) A. DC Determined Using SSR Markers
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Um Yu-Rry
Lee Yi
Jin Mei-Lan
Lee Dae-Young
Lee Jeong-Hoon
Kim Geum-Soog
Kim Chang-Kug
Hong Chang-Pyo
Kim Ok-Tae
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Abstract
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Background : Plant breeding requires the collection of genetically diverse genetic resources. Studies on the characteristics of Platycodon grandiflorum resources have not been carried out so far. The present study was carried out to discriminate P. grandiflorum based on morphological characteristics and genetic diversity using simple sequence repeat (SSR) markers.
Methods and Results : We collected 11 P. grandiflorum cultivars: Maries II, Hakone double white, Hakone double blue, Fuji white, Fuji pink, Fuji blue, Astra white, Astra pink, Astra blue, Astra semi-double blue and Jangbaek. Analyses of the morphological characteristics of the collection were conducted for aerial parts (flower, stem and leaf) and underground parts (root). Next, the genetic diversity of all P. grandiflorum resources was analyzed using SSR markers employing the DNA fragment analysis method. We determined that the 11 P. grandiflorum cultivars analyzed could be classified by plant length, leaf number and root characteristic. Based on the genetic diversity analysis, these cultivars were classified into four distinct groups.
Conclusions : These findings could be used for further research on cultivar development using molecular breeding techniques and for conservation of the genetic diversity of P. grandiflorum. Moreover, the markers could be used for genetic mapping of the plant and marker-assisted selection for crop breeding.
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KEYWORD
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Platycodon grandiflorum, Morphological Characteristics, Genetic Diversity, Simple Sequence Repeats
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